Protein microarray:


Protein microarray:

  • Protein microarrays, also known as protein chips, are miniaturized, parallel assay systems that contain small amounts of purified proteins in a high-density format. They allow the simultaneous determination of a variety of analytes from small amounts of samples in a single experiment.

Advantages/Application of protein microarray:
Protein-Protein and Protein-Lipid Interactions:
  • These proteome chips were first used to study protein- protein interactions, with the chips being incubated with biotinylated calmodulin in order to identify new binding partners.
  • In addition, protein microarrays have also been used to examine interactions with various phospholipids that are known to act as secondary messengers.
  • When biotinylated liposomes containing various phospholipids of interest were used as probes, more than 150 phospholipid binding proteins were identified, and a wide range of proteins was found to bind to the lipid vesicles.
Protein-Small Molecule Interactions
  • Protein microarrays also have great potential for use in drug discovery and the identification of drug targets.
  • When performed on a proteome chip, the binding profile of a drug of interest can be simultaneously obtained across an entire proteome and allows for monitoring its specificity and/or off-target effects.


Protein-Peptide Interactions
They could measure quantitative interactions between proteins and peptides in an array format. Protein-DNA Interactions
Such protein-DNA binding information is necessary for uncovering gene regulatory networks that control cellular and developmental processes yeast proteome chips to screen for novel DNA binding activities using fluorescently labeled yeast genomic DNA.
Identification of Kinase Substrates on Protein Chips
Since phosphorylation is known to be involved in almost every aspect of cell processes, identification of the downstream substrates of protein kinases is a critical step in understanding the effects of phosphorylation on protein functions.
Protein Glycosylation Analysis
Glycosylation is one of the most important protein posttranslational modifications and is highly related to the regulation and function of many proteins.
Profiling Immune Responses
The microarray-based identification of the autoantigens targeted by autoantibodies during the immune response has considerable potential for use in diagnosis, classification, and prognosis.
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